Flottilin antibody
WebAll lanes : Anti-Flotillin 1 antibody [EP446Y] (ab78178) at 1/1000 dilution Lane 1 : Wild-type HEK293T cell lysate Lane 2 : FLOT1 knockout HEK293T cell lysate Lane 3 : A431 cell lysate Lane 4 : HAP1 cell lysate Lysates/proteins at 20 µg per lane. Secondary All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed at 1/10000 dilution Predicted … WebRecombinant Anti-Flotillin 1 antibody [EPR6041] ab133497 is a primary antibody from Abcam. 57 Citations Host: Rabbit Clonality: Recombinant (EPR6041) Validations: Knockout. Applications: WB, FC/FACS and 5 more Reactivity: Homo sapiens (Human), Mus musculus (House mouse), and Rattus norvegicus (Rat)
Flottilin antibody
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WebHuman Flotillin-1 recombinant protein corresponding to amino acids 1-270 expressed in E. coli. Formulation. This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives. Preparation. The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
WebAnti-Flotillin 1 antibodies are available from several suppliers. In humans, this protein is encoded by the gene FLOT1. The protein may also be known as integral membrane component of caveolae. The expected protein mass is 47.4 kDa, but there are 2 reported isoforms. Variants of this antigen may also be found in other species, including plant ... WebWestern blot of anti-Flotillin-1 antibody (clone W16108A) and isotype-matched IgG2a control. Blots were incubated with 5µg/ml (left) or 0.5µg/ml (middle) anti-Flotillin-1 antibody, or rat IgG2a, κ isotype control antibody (right) overnight at 4°C, followed by the incubation with horseradish peroxidase labeled goat anti-rat secondary antibody.
WebRed blood cell antibody identification: Single antibody specificity; Multiple antibody specificities; Antibody to high prevalence antigen; Antibody to low prevalence antigen; … WebWestern blot of anti-Flotillin-1 antibody (clone W16108A) and isotype-matched IgG2a control. Blots were incubated with 5µg/ml (left) or 0.5µg/ml (middle) anti-Flotillin-1 …
Webidentity and significance of flotillin cross-reacting determinant remains unknown. Here, we examine the cell type and tissue-specific expression of the flotillins using a novel monoclonal antibody probe that recognizes only flotillin-1. A survey of cell and tissue types demonstrates that flotillins 1 and 2 have a complementary tissue distribution.
WebBiochem/physiol Actions. Flotillin-2 is a protein encoded by the FLOT2 gene in humans. Flotillins are involved in cell adhesion processes and play a role during breast cancer progression. FLOT2 especially plays a functional role in cell-cell adhesion in non-malignant epithelial cells. 6] It may have an essential function in intestinal ... supply reliability definitionWebFlotillin-2 is mainly localized to the plasma membrane and is prevalent in cell-cell contact sites. However, overexpressed flotillin-2 has also been found in the late endosome (4,8,9). Both flotillin-1 and flotillin-2 are … supply relay for a refrigerator compressorWebGeneral notes. This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility. - Improved sensitivity and specificity. - Long-term security of supply. - Animal-free production. For more information see here. Our RabMAb ® technology is a patented hybridoma ... supply remote jobsWebFlotillin 1 Antibody (PA5-17127) in WB. Western blot was performed using anti-Flotillin 1 Polyclonal Antibody (Product # PA5-17127) and a 47kDa band corresponding to Flotillin 1 was observed across all the cell lines and tissue lysates tested except THP1 which is reported negative for Flotillin 1 expression. Whole cell extracts (30 µg lysate ... supply reliabilityWebWestern blot was performed using Anti- Flotillin 1 Polyclonal Antibody ( Product # PA5-19713) and a 47kDa band corresponding to Flotillin 1 … supply reliability dashboardWebThe blot was probed with Flotillin 2 Polyclonal Antibody (Product # PA5-30033, 1:500 dilution) and Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 … supply repuestosWebIn addition to its colocalization with lipid rafts on the plasma membrane, flotillin-1 also has been found in compartments of the endocytic and … supply removal trap gw2