WebMay 15, 2006 · This DNA fragment was cloned into a T-vector (TaKaRa, Japan), verified by sequencing, and inserted into the plant transformation vector pMON530 (Monsanto, USA), downstream to the 35S promoter. WebNov 18, 2014 · As a control, vector DNA containing the CaMV 35S promoter alone (pMON530) was introduced into Agrobacterium. Agrobacterium infusion assays and RNA isolation. Vacuum infiltration of Arabidopsis thaliana plants with Agrobacterium cultures was performed essentially as described .

CKI1, a Histidine Kinase Homolog Implicated in Cytokinin

WebAgrobacterium-mediated transformation is the preferred method to transform plants because it usually results in simple insertions of target DNA. In this article, we describe a method for Agrobacterium-mediated transformation of the inbred B. distachyon lines Bd21 and Bd21-3. Embryogenic callus induced from immature embryos is co-cultivated with ... Webrived from the binary vector pMON530 [15]. In designing the T-DNA of the binary vector the primary aim was to provide an organisational structure with a IacZ' region immediately 3' of the right T-DNA border and 'overdrive' enhancer … pcl bg8002

The AP2/ERF transcription factor - ScienceDirect

WebJan 14, 2010 · The resulting plasmid pMON530-CLS was first introduced into Agrobacterium tumefaciens by electroporation and then transformed into the Arabidopsis las mutant by floral dip method (Clough and Bent 1998). Transgenic plants were selected on MS medium containing 20 mg/L Kanamycin. DNA Isolation and Gel Blot Analysis WebApr 28, 2015 · The final construct 35S:EYFP-LeNRT2.3/pMON530 and the empty vector 35S:EGFP/pMON530 were transiently expressed in onion epidermal cells using a particle gun-mediated system (PDS-1000/He; Bio-Rad). The bombarded cells were held in the dark at 22 °C for 12 h followed by YFP imaging using confocal microscopy (Carl Zeiss; LSM … WebMay 30, 1993 · These DNA fragments have been cloned into the binary vector, pMON530, such that either the nopaline synthase (Nos) promoter or cauliflower mosaic virus (CaMV) 35S RNA promoter is used to drive synthesis of the corresponding sense and AS RNAs. Transgenic Nicotiana tabacum cv. Xanthi nn plants containing these constructs were … pclbghnu